Yes, St. Croix and the wider U.S. Virgin Islands are home to several species of tarantulas, though they are generally shy and not dangerous to humans. The most common species is the Cyrtopholis bartholomaei, often referred to locally as the "ground spider." These tarantulas typically live in small, silk-lined burrows in the soil or under rocks and are most active at night. While their large, hairy appearance can be startling to tourists, they are not aggressive and their venom is comparable to a mild bee sting. Another species occasionally found in the region is the Puerto Rican Pinktoe (Caribena laeta), which is more arboreal, meaning it lives in trees and shrubs. You are unlikely to encounter one in your hotel room; they prefer the humid, forested areas of the island's West End or hiking trails like the Jack and Isaac Bay Preserve. In the local ecosystem, these spiders are considered beneficial "natural pest control," as they feed on cockroaches, crickets, and other insects that would otherwise proliferate in the tropical climate.