Yes, commercial and private pilots are allowed to wear glasses while on the job. To fly as a commercial or private pilot, you must first pass a medical exam given by an Aviation Medical Examiner (AME). The exam includes a vision screening and other medical evaluations.
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Commercial pilots will be allowed tattoos in some airlines as long as they are covered. This means if a pilot has a sleeve-type tattoo on their arm, they must wear a long sleeve shirt to cover it. Most major airlines do not allow tattoos that cannot be covered.
In the U.S., there are no FAA age limits for pilots except for commercial airline pilots employed by airlines certificated under 14 CFR Part 121. These airlines cannot employ pilots after they reach the age of 65. However, these pilots may stay on with a Part 121 carrier in some other role, such as flight engineer.
Most female pilots in fact do and can wear whatever they'd like. I often have my nails done. Acrylics, powder, or just plain gel. The FA manuals typically provide more guidance that it must be professional/not chipped, but it varies per company.