Yes, you can generally check in at 2:00 AM, provided the hotel offers 24-hour reception and you have notified them of your late arrival in advance. Most major international chains and full-service hotels in urban or airport locations are staffed around the clock specifically to accommodate travelers with delayed flights or red-eye schedules. However, it is a critical "pro-traveler" rule to call or message the hotel if you expect to arrive after midnight; without this notification, a hotel may mark you as a "no-show" and release your room to another guest during their nightly system audit (often performed between 1:00 AM and 3:00 AM). For smaller boutique hotels, motels, or bed-and-breakfasts with limited front desk hours, a 2:00 AM arrival may require a self-check-in procedure, such as a lockbox code or a digital key provided via a mobile app. Always remember that checking in at 2:00 AM still counts as the night before—meaning you must have booked the room starting the previous day to have it ready upon your arrival, as standard "morning" check-in doesn't usually begin until 3:00 PM.