Temperature of the water in Jacob's Well remains a near-constant 68°F and visibility is typically excellent. The spring provides habitat to numerous species of fish (principally sunfish and perch); turtles; aquatic insects; crustaceans; and, most notably, the cave-adapted Fern Bank salamander (Eurycea pterophilia).
As of June 29, 2022, swimming will not be allowed at Jacob's Well Natural Area (JWNA) for the foreseeable future. The threat of high bacteria levels, other pollutants and poor visibility conditions are unsafe for swimming.