Yes, you can visit both Pisa and Cinque Terre in a single day from Florence, but it is a very long and structured 12-to-13-hour journey. In 2026, many tour operators offer small-group van tours that start early (around 7:30 AM), stopping in Pisa for about an hour to see the Leaning Tower before heading to La Spezia, the gateway to the Cinque Terre. If traveling independently by train, you would take a morning train to Pisa Centrale, spend 90 minutes at the Piazza dei Miracoli, and then take a direct train to La Spezia to board the Cinque Terre Express. Because the five villages are connected by very short train rides (often just 4-6 minutes apart), you can realistically explore two or three of the towns—such as Vernazza and Manarola—before needing to head back to Florence. While rewarding, this itinerary is exhausting and only allows for a "highlight reel" experience, so it is best suited for travelers who are short on time and don't mind a fast-paced day of walking and train transfers.