Yes, you can stay overnight at the Toronto Zoo through their popular "Serengeti Bush Camp" and "Pyjama Party" programs. The Serengeti Bush Camp offers an authentic-feeling overnight experience in the heart of the zoo’s African Savanna, where guests sleep in canvas tents and participate in guided night tours and campfire activities. For 2026, the zoo also offers seasonal "overnight safaris" for families and groups, which include dinner, breakfast, and exclusive behind-the-scenes access to animal habitats. These programs are highly sought after and often sell out months in advance. While there isn't a traditional "luxury hotel" on the property, these educational sleepovers provide a unique opportunity to hear the lions roar and the zebras call while the rest of the city is asleep, making it one of the most immersive wildlife experiences in Canada.