Yes, you can absolutely stay in Tuscany without a car, and doing so often leads to a more "authentic" and relaxed experience. The key is to base yourself in major hubs like Florence, Siena, or Pisa, which are exceptionally well-connected by Italy’s Trenitalia rail network. From Florence, you can reach Lucca, Arezzo, and even smaller gems like Pistoia with ease. For the iconic hilltop villages not served by trains, such as San Gimignano or Pienza, the "Autolinee Toscane" bus system provides an affordable alternative, though it requires more careful schedule planning. In 2026, many agriturismos (farm stays) located near train stations offer pick-up services for guests. While the rolling hills of the Val d'Orcia are more challenging to navigate via public transit, many travelers opt for guided day tours from Siena to bridge the gap. Staying in a central city allows you to enjoy the evening "passeggiata" and local wines without worrying about navigating the region's notoriously narrow and winding roads or the restricted "ZTL" driving zones.