Yes, every Greyhound bus is equipped with an on-board toilet, located at the rear of the vehicle. While Greyhound makes regular stops at official stations and rest areas for "comfort breaks," the on-board facility provides a necessary option for passengers during long-distance journeys. In 2026, the upgraded fleet features restrooms that are designed to be more hygienic and easier to maintain, though their cleanliness still depends heavily on the duration of the trip and the number of passengers using them. It is generally recommended to use the facility for emergencies and take advantage of the more spacious restrooms at scheduled stops. In addition to the bathroom, modern Greyhound buses offer other amenities such as free Wi-Fi, individual power outlets at nearly every seat, reclining leather chairs, and extra legroom, as the company has recently removed a row of seats from its standard configuration to improve passenger comfort.