Yes, New York City has one of the most extensive 24-hour bus networks in the world. Most of the Metropolitan Transportation Authority (MTA) bus routes in all five boroughs operate 24 hours a day, 7 days a week, including holidays. While the frequency of the buses drops significantly during the "late-night" hours (typically between midnight and 5:00 AM), you can still find service on major thoroughfares. During these off-peak hours, some routes may run every 20 to 60 minutes, so it is crucial to use real-time tracking apps like the MYmta app or Google Maps to check the exact arrival times. Additionally, the MTA operates a specific network of "Select Bus Service" (SBS) and "Express" buses, though Express bus schedules are more limited and often do not run 24/7. One helpful tip for night travelers is the "Request-a-Stop" program: between 10:00 PM and 5:00 AM, passengers can ask the driver to let them off at a safe location along the route that is not an official bus stop. This feature is designed to enhance passenger safety and convenience during the quietest hours of the night in the city that never sleeps.