Yes, taxis in major Italian cities like Rome, Milan, and Florence generally run 24/7, but finding one on the street at 3:00 AM can be difficult. In Italy, you cannot usually "hail" a moving taxi; instead, you must go to a designated "Taxi Rank" (posteggio dei taxi) or call for one via a radio taxi service. At night, most drivers wait at these ranks, often found near major piazzas, train stations, and airports. For early morning departures, it is highly recommended to book a taxi in advance using popular apps like Free Now (itTaxi) or by having your hotel concierge call a local dispatcher. Be aware that nighttime fares (typically starting after 10:00 PM) incur a surcharge, and the meter will start running from the moment the driver accepts the call to come pick you up. Always ensure you are using an official, licensed white taxi with a "Taxi" sign on top and a meter clearly visible to avoid being overcharged by unlicensed "NCC" drivers.