Yes, the Hogwarts Express at Universal Orlando Resort travels both ways between Diagon Alley (Universal Studios Florida) and Hogsmeade (Universal Islands of Adventure). However, it is important to note that the experience is completely different depending on which direction you are traveling. When going from London to Hogsmeade, the story focuses on leaving the Muggle world, featuring cameos from characters like Hagrid and views of the English countryside. Conversely, the trip from Hogsmeade to London features different projections and audio, including Fred and George Weasley and a more urban approach to King's Cross. To ride the train, guests must have a "Park-to-Park" ticket, as it serves as a functional transport between two distinct theme parks.