No, the Lisbon Metro (Metropolitano de Lisboa) does not run 24/7 in 2026. Under normal operating conditions, all four lines (Blue, Yellow, Green, and Red) open at 06:30 AM and close at 01:00 AM every day, including weekends and public holidays. If you need to travel across the city between 01:00 AM and 06:30 AM, you must rely on the "Rede da Madrugada" (Night Bus Network) operated by Carris, or use rideshare services like Uber and Bolt, which are very active in Lisbon. In 2026, the only exceptions to the 01:00 AM closure are typically New Year's Eve or major city festivals like the Santo António (June 12-13), where specific lines may run all night to accommodate the crowds. For a supportive tip: if you are landing at Lisbon Airport after midnight, the Red Line metro may still be running if you hurry, but if you land after 12:45 AM, you should head straight for the taxi rank or bus stop.