Yes, TSA regulations require that all pets be removed from their carrying cases so that the carrier can be passed through the X-ray machine. You should never send your cat through the X-ray tunnel. When you reach the front of the security line, you will be asked to take your cat out, hold them in your arms, and walk through the metal detector. Because airports are loud and high-stress environments, many cats may try to bolt; it is highly recommended to have your cat in a harness with a leash even while they are inside the carrier so you have a secure grip on them during the walk-through. If you are concerned about your cat escaping or becoming aggressive, you can request a private screening room. In this case, a TSA officer will take you and your pet to a secure, enclosed area to perform the inspection. After the carrier is cleared and the cat is back inside, you are free to proceed to your gate.