The flow of the spring is the fourth highest among all the springs in Florida. The cool, clear waters of the headsprings are incredibly refreshing place to swim. The average depth in the natural swimming area runs from 10 to 18 feet, and the water temperature averages 72 degrees year-round.

The water in Florida's Rainbow River maintains a remarkably constant temperature of approximately 72°F (22°C) throughout the entire year. This consistency is due to the river being fed by first-magnitude springs that originate from deep within the Floridan Aquifer. Because the water comes from underground, it is insulated from the seasonal changes in air temperature, meaning it feels refreshingly cool during the hot Florida summers and surprisingly warm on crisp winter days. While 72 degrees is perfect for swimming, snorkeling, and tubing, it can feel quite chilly if you are submerged for an extended period without much physical activity. Many regular visitors wear a thin "shorty" wetsuit or a rash guard to stay comfortable during a two-hour tube float down the river. The crystal-clear visibility, often exceeding 100 feet, makes it one of the most popular spots in the state for observing aquatic life like turtles, otters, and various species of fish in their natural habitat.