The Milan metro runs every day from 6 am until 12:30 am (midnight). On 25 December and 1 May, the metro opens from 7 am until 7:30 pm. The frequency of the Milan Metro depends on the time of day, on the line, and the day of the week, but generally, the trains arrive every 2-4 minutes.

Generally, the Milan Metro (Metropolitana di Milano) closes around midnight or 12:30 AM on weekdays. However, for the 2026 Milan-Cortina Winter Olympics, the city has implemented extraordinary measures to support nighttime mobility. During the Olympic period (February 5–22, 2026), metro service is extended until 2:00 AM across the urban network to accommodate the large influx of spectators and athletes. Outside of special events like the Olympics or New Year's Eve, the metro is replaced late at night by a robust "NM" (Notte) surface bus network. These 15 night bus lines (NM1, NM2, NM3, etc.) follow the same routes as the metro lines and run throughout the early morning hours, typically from 1:30 AM to 5:30 AM, ensuring that residents and tourists can still navigate the city after the trains have stopped. In 2026, the city is also focusing on "green mobility," offering free public transport for Olympic stakeholders and expanding dedicated lanes for the iconic 90-91 trolleybus line to maintain transit reliability during the Games.