Riders sit in vehicles modeled after karts from the game and wear VR headsets. The ride can only accommodate people who have a 40-inch waist or smaller, according to safety rules published by Universal Studios Hollywood.

The Mario Kart: Bowser’s Challenge ride at Super Nintendo World (at both Universal Studios Hollywood and Japan) does not have a "waist limit" measured in inches, but it does have a "test seat" policy for guests with a waistline of 40 inches (102 cm) or more. The ride vehicles use a lap bar restraint system that must be able to click into a specific safety position to secure the rider. Because of the "tight" design of the Mario Kart vehicles, guests with larger frames or broad shoulders may find the seating restrictive. Universal explicitly encourages guests whose waistline is 40 inches or larger to use the test seat located at the attraction's entrance before joining the queue. It is important to note that the limit is based on body shape and how you sit rather than just a number on a scale; many guests with a 40-inch waist fit comfortably, while others may struggle if their weight is distributed in a way that prevents the lap bar from locking.