Delta Air Lines is currently the largest operator with 65 airplanes in its fleet. As of September 2023, A330 orders stood at 1,809 of which 1,582 had been delivered and 1,466 were in service with 145 operators. The global A330 fleet had accumulated more than 60 million flight hours since its entry into service.

As of 2026, Delta Air Lines is the largest operator of the Airbus A330 family in the world, particularly when focusing on the modern A330neo (A330-900) variant. Delta has integrated over 80 A330 aircraft into its fleet, using them as the backbone of its trans-Atlantic and trans-Pacific operations. Other massive operators include Turkish Airlines, which maintains a huge fleet of both the A330-200 and A330-300 versions to serve its vast global network from Istanbul. In the Asian market, China Eastern Airlines and Air China remain top-tier operators with dozens of these aircraft in active service. The A330 remains popular because it offers a "sweet spot" of capacity and range that is more economical for certain routes than the larger A350. While many airlines are transitioning to newer models, the reliability and refurbished cabins of the A330 fleet keep these carriers at the top of the list for total airframes managed.

As of the most recent data, Turkish Airlines is the largest operator of the Airbus A330 in the world. The airline operates a large fleet of A330s, including both the A330-200 and A330-300 variants. Turkish Airlines utilizes these aircraft extensively for its long-haul and medium-haul routes, connecting Istanbul to destinations across Europe, Asia, Africa, and the Americas.

Other major operators of the A330 include Delta Air Lines, China Eastern Airlines, and Cathay Pacific, but Turkish Airlines leads in terms of fleet size and utilization of the A330 family.