While many bus operators suspend services after midnight, there are select DC Circulator and Metrobus routes that provide late night service on specific routes.
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Under the current operating schedule, buses are supposed to (but do not always) run every 12 minutes or less from 7 a.m. to 9 p.m. Buses are then scheduled to offer service every 30 minutes until 2 a.m. They currently do not offer service from 2 a.m. to 4 or 5 a.m., depending on the route.
Many of London's bus routes run 24/7.When the Underground closes between about midnight and about 5am, extra night buses are put on. In the centre of London you only wait a few minutes for a bus whatever time of day or night.
The Washington Metropolitan Area Transit Authority (WMATA) provides bus and rail transit service to Washington, DC and the Maryland and Virginia suburbs. Metrobus operates 24-hours-a-day, 7 days a week with approximately 1,500 buses. Service intervals vary by time of day and by weekday/weekend to meet demand.
Metrorail, Metrobus and the DC CirculatorTrips are paid through a SmarTrip account, either by a reloadable plastic card or mobile pay. This national capital area system includes Metrorail, Metrobus and the DC Circulator. For specific directions in utilizing WMATA services, check out their Trip Planner.
The best way to get around Washington DC is the DC Metro (subway or underground). You can find a metro map of all the different lines when you first enter the metro station and online.
The only down side is the trains run a little less frequently at that time of night, but it is perfectly safe. I agree with MikeT. My friend and I (women in their 20's) felt completely safe taking the Metro at night. Enjoy DC!