No, standard buses in Tokyo are not 24-hour services. Most Toei and private bus lines in central Tokyo begin service around 6:00 AM and end between 10:00 PM and midnight. While Tokyo is a "non-stop" city, the public transport network—including the subways and most buses—shuts down for a few hours in the middle of the night for maintenance. However, there are a limited number of "Midnight Buses" that operate on certain popular routes (often departing from major hubs like Shibuya or Shinjuku) until about 1:00 AM or 2:00 AM, though these charge double the standard fare. For 24-hour transit in 2026, travelers almost exclusively rely on taxis or rideshare services like S.Ride or Uber. During the New Year's Eve period, some specific lines may operate all-night services, but for the typical 2026 traveler, the "last bus" is a critical deadline to keep in mind.