Yes, trains are legally required to sound their horns regardless of the time of day when approaching public highway-rail grade crossings. According to the Federal Railroad Administration (FRA) "Train Horn Rule," locomotive engineers must sound the horn in a specific pattern—two long, one short, and one long blast—at least 15 to 20 seconds before reaching a crossing. This is a critical safety measure intended to warn pedestrians and motorists of the train's approach. While many communities in 2026 have established "Quiet Zones" where routine honking is prohibited at night, engineers still retain the ultimate authority to sound the horn if they perceive a safety hazard, such as an animal, vehicle, or person on the tracks. These safety requirements prioritize the prevention of collisions over noise abatement, even during late-night or early-morning hours.