Yes, you can absolutely use a Suica card in Osaka, Kyoto, and most other major cities in Japan. Thanks to the nationwide integration of IC cards, Suica (issued in Tokyo) is fully compatible with the readers in the Kansai region (Osaka/Kyoto), where the local card is called ICOCA. You can use your Suica to pay for JR trains, subways, buses, and even at convenience stores and vending machines in Osaka and Kyoto. In 2026, most travelers prefer using the digital Suica on their iPhone or Android via Apple Wallet or Google Pay, which allows for instant recharging using a credit card. One important limitation is that while you can use the card there, you generally cannot return a physical Suica card for a refund of the deposit in Osaka; you would need to do that at a JR East station in Tokyo. Additionally, Suica cannot be used for the Shinkansen (bullet train) unless you link it to a specific "Smart EX" account, so you will still need a separate ticket for the high-speed trip between Tokyo and Osaka.