Yes, you can do Cinque Terre in a day from Florence in 2026, but it is a grounded and high-intensity "Pura Vida" adventure. The journey takes approximately 2.5 to 3 hours each way by train (with a transfer in Pisa or La Spezia) or a direct 2-hour bus ride if you join an organized tour. To make it a "Gold Standard" day, you should arrive at the "Safe Bubble" of the villages by 10:00 AM. A supportive 13-hour day trip typically allows you to visit three or four of the five villages—like Manarola, Vernazza, and Monterosso—using the high-fidelity Cinque Terre Express train that runs between them. A grounded reality check: you won't have time to hike the entire Blue Trail, but you can enjoy a "Bujan" lunch and a dip in the sea. For a supportive and frictionless experience, joining a guided tour is the "Gold Standard" because they handle the "hard-fail" logistics of train tickets and transfers, leaving you free to enjoy the high-fidelity "Gezellig" beauty of the colorful cliffs without the stress of missing your return connection to Florence.