Yes, you can use a Universal Express Pass for the Hogwarts Express at Universal Orlando Resort. Because the train is an immensely popular attraction that transports guests between Universal Studios Florida (King's Cross Station) and Islands of Adventure (Hogsmeade Station), lines can often exceed an hour. Having an Express Pass allows you to enter a dedicated, shorter queue, which is particularly helpful if you are trying to maximize your time in both parks. However, the most critical requirement for riding the Hogwarts Express is not the Express Pass, but a Park-to-Park ticket. Because the train moves between two separate theme parks, the conductors will scan your ticket before you enter the queue; if you only have a single-park ticket, you will not be allowed to board the train regardless of whether you have an Express Pass or not.