Yes, visiting the Cinque Terre from Tuscany is a very popular and feasible day trip, particularly if you are based in Florence, Lucca, or Pisa. The most efficient way to get there is by train. From Florence, the journey takes about 2.5 to 3 hours, requiring a change at Pisa or La Spezia. Once you arrive at La Spezia Centrale, you can purchase a "Cinque Terre Card" which gives you unlimited access to the regional trains that hop between the five colorful villages (Riomaggiore, Manarola, Corniglia, Vernazza, and Monterosso) in just a few minutes each. Driving from Tuscany is generally discouraged, as the villages are mostly car-free and parking in the gateway towns is extremely limited and expensive. For a more scenic experience, you can take a ferry from the La Spezia waterfront, which allows you to see the stunning coastline from the sea—the best angle for photography. While it is a long day (expect about 12 hours total for a round trip from Florence), it is entirely possible to see three or four of the villages in one go if you start early. Just be prepared for significant walking and many stairs, as the terrain is very steep and rugged.