Usually found at the front or back of each train car, they include a sink, toilet and locking door. All High-Speed trains are fully heated and air-conditioned. For safety reasons, high-speed trains do not offer the option of opening windows.

Yes, almost all Trenitalia trains are equipped with onboard toilet facilities, though the quality and quantity vary significantly by the type of service. On high-speed Frecciarossa and Frecciargento trains, you will find modern, accessible, and generally well-maintained vacuum toilets in every carriage, usually located near the vestibules. These often include baby-changing tables and touchless soap dispensers. On Intercity trains, toilets are standard but may be slightly older in design. Even on Regionale and the newer "Pop" or "Rock" regional trains, toilets are a mandatory feature. However, travelers should be aware that on some very old regional stock, the toilets may still utilize "drop pipes" (though these are being phased out in 2026), and it is a long-standing Italian tradition that toilets may be closed while the train is stopped at a station. It is always wise to carry a small pack of tissues or hand sanitizer, as supplies can occasionally run low during long journeys across the Italian peninsula.