Private water taxis in Venice operate 24 hours a day, 7 days a week, providing a premium door-to-door service that remains available even after public transit options slow down. While they are always available via phone or app, you can typically find them stationed at major hubs like Marco Polo Airport, the Santa Lucia train station, and the Piazzale Roma car park throughout the night. It is important to distinguish these from the Vaporetto (public water bus), which follows a specific schedule; the standard Vaporetto lines generally run until around midnight, after which the "N" (Night) lines take over from approximately 11:30 PM to 5:00 AM, running at 20-to-30 minute intervals. If you are staying at a hotel deep in the canal network, a private water taxi is often your only direct option for a late-night return, though you should be prepared for a night surcharge which typically applies to journeys made between 10:00 PM and 7:00 AM.