How late do water buses run in Venice? Approximately about midnight. Three nighttime routes run from 11:30 p.m. to 5 a.m., while the main routes run from 5 a.m. to midnight.

Yes, the water bus system in Venice, known as the Vaporetto (operated by ACTV), operates 24 hours a day, though the frequency and routes change significantly after dark. During the daytime, dozens of lines crisscross the Grand Canal and the lagoon, but around midnight, the system transitions to the "N" (Notturno) Line. The Night Line typically runs from roughly 11:30 PM until 5:00 AM. It follows a simplified route that starts at San Zaccaria (near St. Mark's Square), travels down the Grand Canal to the Rialto and the Railway Station (Santa Lucia), and extends to the island of Giudecca and the Lido. While daytime boats run every 10–12 minutes, the Night Line usually departs every 20 to 40 minutes. In 2026, tickets and passes (like the Venezia Unica card) are valid for both day and night services. This 24-hour connectivity is essential for travelers with early morning flights from Marco Polo Airport, as they can take the Vaporetto to Piazzale Roma and then catch the "Aerobus" to the terminal, even in the middle of the night.