Private water taxis (motoscafi) in Venice do not actually stop running; they operate 24 hours a day, 7 days a week. However, there are significant price differences depending on when you travel. A "night surcharge" of approximately €15 to €20 is typically applied to journeys taken between 10:00 PM and 7:00 AM. While the taxis are always available, you may find fewer of them waiting at designated stands during the late-night hours, so it is highly recommended to book in advance via a service like Consorzio Motoscafi Venezia if you have a late-night arrival at Marco Polo Airport or a very early morning departure. This distinguishes them from the public water buses (vaporetti), which transition to a limited "Line N" (Night) service after midnight. The private water taxis are the most convenient way to reach your hotel directly, as they can navigate the smaller canals that the large public boats cannot. Just be prepared for the high cost, which for a standard daytime trip often starts at €120 to €150 for up to six people with luggage.