Yes, traveling from Florence (Firenze Santa Maria Novella) to the Cinque Terre is a very popular and straightforward journey by rail. There are no direct high-speed trains that go straight into the five villages, so you will typically take a regionale or Frecciargento train from Florence to La Spezia Centrale, which is the gateway city. This initial leg takes between 1 hour 30 minutes and 2 hours. Once at La Spezia, you transfer to the Cinque Terre Express, a dedicated shuttle train that runs frequently and stops at all five villages: Riomaggiore, Manarola, Corniglia, Vernazza, and Monterosso. In 2026, it is highly recommended to purchase the "Cinque Terre Card," which includes unlimited train travel between La Spezia and Levanto. The journey is incredibly scenic, especially as you emerge from the tunnels to see the Mediterranean Sea. While you can also reach the villages via Pisa, the route through La Spezia remains the most efficient and common path for tourists looking to explore the Italian Riviera from the heart of Tuscany.