The Resort Monorail runs a continuous loop from the Transportation and Ticket Center to the Polynesian resort, the Grand Floridian resort, the Magic Kingdom, and the Contemporary resort, in that order. The Epcot Monorail runs back and forth between the Transportation and Ticket Center and Epcot.

At Walt Disney World in Florida, the monorail system provides direct transportation to two of the four major theme parks: Magic Kingdom and EPCOT. The system is split into three distinct lines. The Resort Monorail and the Express Monorail both service the Magic Kingdom area, with the resort line making additional stops at the Contemporary, Polynesian, and Grand Floridian resorts, as well as the Transportation and Ticket Center (TTC). To reach EPCOT, guests must transfer at the TTC to the EPCOT Monorail line, which provides a scenic loop through the park before docking at the front entrance. In 2026, the monorail remains a nostalgic and efficient "highway in the sky," though it does not service Disney's Hollywood Studios or Disney's Animal Kingdom, which are instead reached via the Disney Skyliner, buses, or watercraft. At Disneyland in California, the monorail is a single line that travels between Tomorrowland inside Disneyland Park and the Downtown Disney District, effectively serving as a park-to-district shuttle rather than a multi-park transit system.