Yes, almost all GO buses (the regional transit service for the Greater Golden Horseshoe in Ontario, Canada) are equipped with a small, basic on-board restroom. These washrooms are typically located at the rear of the bus on the lower level (for double-decker models) or the main level (for standard motorcoaches). In 2026, GO Transit has continued to upgrade its fleet to ensure that these facilities are clean and accessible, recognizing that many commuters spend over an hour on routes like the 16 or 21. While the buses have bathrooms, GO Transit encourages passengers to use the more spacious public washrooms located at major GO Bus Terminals and train stations, which are maintained to "State of Good Repair" standards. If you are traveling on a shorter local bus route within a specific city (like the TTC in Toronto or MiWay in Mississauga), those buses do not have bathrooms, but the regional GO coaches are specifically designed for "inter-city" comfort, much like a Greyhound or Megabus, ensuring that "nature's call" doesn't disrupt your commute across the GTHA.